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dmem 048– 29763  (FUJIFILM)

 
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    Structured Review

    FUJIFILM dmem 048– 29763
    Dmem 048– 29763, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dmem+048%E2%80%93+29763/amino+acid+free+dmem/pm32048867-171-8-13
    Average 90 stars, based on 1 article reviews
    dmem 048– 29763 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
    Article Snippet: Freshly isolated gastrocnemius muscles were incubated in a permeabilization buffer [DMEM (048– 29763, Fujifilm Wako Pure Chemical), 2% horse serum (16050122, Thermo Fisher Scientific), and 0.1% saponin (30502–42, Nacalai Tesque)] with 5 g/mL Hoechst 33342 (nuclear dye; Ex: 342 nm; Em: 461 nm; 346–07951, Dojindo Molecular Technologies) and 200 nM MitoBrightRed (membrane potential-dependent mitochondrial dye; Ex: 547 nm; Em: 563 nm; MT-7, Dojindo Molecular Technologies) for 20 min at room temperature with shaking.

    Muscles:

    Article Title: Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
    Article Snippet: Freshly isolated gastrocnemius muscles were incubated in a permeabilization buffer [DMEM (048– 29763, Fujifilm Wako Pure Chemical), 2% horse serum (16050122, Thermo Fisher Scientific), and 0.1% saponin (30502–42, Nacalai Tesque)] with 5 g/mL Hoechst 33342 (nuclear dye; Ex: 342 nm; Em: 461 nm; 346–07951, Dojindo Molecular Technologies) and 200 nM MitoBrightRed (membrane potential-dependent mitochondrial dye; Ex: 547 nm; Em: 563 nm; MT-7, Dojindo Molecular Technologies) for 20 min at room temperature with shaking.

    Incubation:

    Article Title: Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
    Article Snippet: Freshly isolated gastrocnemius muscles were incubated in a permeabilization buffer [DMEM (048– 29763, Fujifilm Wako Pure Chemical), 2% horse serum (16050122, Thermo Fisher Scientific), and 0.1% saponin (30502–42, Nacalai Tesque)] with 5 g/mL Hoechst 33342 (nuclear dye; Ex: 342 nm; Em: 461 nm; 346–07951, Dojindo Molecular Technologies) and 200 nM MitoBrightRed (membrane potential-dependent mitochondrial dye; Ex: 547 nm; Em: 563 nm; MT-7, Dojindo Molecular Technologies) for 20 min at room temperature with shaking.

    Membrane:

    Article Title: Acetaldehyde dehydrogenase 2 deficiency increases mitochondrial reactive oxygen species emission and induces mitochondrial protease Omi/HtrA2 in skeletal muscle.
    Article Snippet: Freshly isolated gastrocnemius muscles were incubated in a permeabilization buffer [DMEM (048– 29763, Fujifilm Wako Pure Chemical), 2% horse serum (16050122, Thermo Fisher Scientific), and 0.1% saponin (30502–42, Nacalai Tesque)] with 5 g/mL Hoechst 33342 (nuclear dye; Ex: 342 nm; Em: 461 nm; 346–07951, Dojindo Molecular Technologies) and 200 nM MitoBrightRed (membrane potential-dependent mitochondrial dye; Ex: 547 nm; Em: 563 nm; MT-7, Dojindo Molecular Technologies) for 20 min at room temperature with shaking.



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